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. Author manuscript; available in PMC: 2009 May 1.
Published in final edited form as: Matrix Biol. 2008 Jan 16;27(4):330–338. doi: 10.1016/j.matbio.2008.01.002

Figure 4. Col11a1 antisense morpholinos induce increased expression of osteoblast markers.

Figure 4

A. C2C12 cells were treated with either no (left column), control (center column) or Col11a1 antisense morpholinos (right column) and immunofluorescently stained with an anti-Col11a1 primary antibody and a TRITC-labeled secondary antibody. C2C12 cells were induced to differentiate into the osteoblastic lineage by treatment with BMP-2 for the indicated length of time. B. C2C12 cells were treated either with no (A), control (B) or Col11a1 antisense morpholinos (C) and stained for alkaline phosphatase activity (red) and mineralization (von Kossa staining, black) after 14 days treatment with BMP-2.