Skip to main content
. Author manuscript; available in PMC: 2009 May 1.
Published in final edited form as: Matrix Biol. 2008 Jan 16;27(4):330–338. doi: 10.1016/j.matbio.2008.01.002

Figure 5. Alkaline phosphatase expression is inhibited during osteoblast differentiation by an N-terminal Col11a1 fragment, V1B.

Figure 5

C2C12 cells were differentiated in osteogenic medium supplemented with BMP2 for five days (A) and MC3T3-E1 cells were differentiated in osteogenic medium for nine days (B) in the presence of vehicle alone, Col11a1 V0 fragment, or Col11a1 V1B fragment. RNA was collected at the indicated time points and quantitative PCR was performed using alkaline phosphatase and actin specific primers. Alkaline phosphatase values were normalized to actin.