Figure 3.

PCR analysis to detect neo^r+ (A) and HBsAg (B) transgenes in DNA extracts of whole blood (mesoderm) and skin (ectoderm) of calves derived from embryos infected with VSV-G-pseudotyped vector. (A and B) Lanes 1–5 are DNA from blood samples and lanes 6–10 are DNA from skin tissue of transgenic calves (lane 1: zygote treatment group, male; lane 2: oocyte treatment group, female; lane 3: oocyte treatment group, female; lane 4: oocyte reatment group, female; lane 5; oocyte treatment group, male.). Lanes 4 and 5 were from twins resulting from implantation of two embryos and the birth of phenotypically distinct calves of different breeds. The negative controls in lanes 11–13 comprise DNA from a blood sample from calf 12, which was a naturally conceived nontransgenic calf, commercial bull semen and ovarian tissue from a cow. Lane 14 contains the plasmid DNA of LSRNL, and lane 15 is a water control. Tissue samples from blood and skin show positive signal with both primer sets, but none of the negative controls show the presence of transgene. Differences in intensity of signal do not reflect copy numbers of the transgene inserted.