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. 1998 Nov 24;95(24):14028–14033. doi: 10.1073/pnas.95.24.14028

Figure 4.

Figure 4

Southern blot analysis of HindIII-digested genomic DNA (A) to detect the 1.6-kb fragment from the HBsAg gene in blood (B) and skin (C) tissue of calves. (B) Lanes 1–5, transgenic calves. Lane 6, nontransgenic calf born after VSV-G-pseudotyped vector injection. Lanes 7 and 8, nontransgenic aborted fetuses. Negative controls include calf 12, which was a naturally conceived nontransgenic calf, ovarian tissue from a cull cow and commercial bull semen. (C) Lanes 1–5, transgenic calves. Lane 6, nontransgenic calf. Negative controls, calf 12 (naturally conceived nontransgenic). Cows 1–4 were the recipients carrying the transgenic calves. HindIII-digested pLSRNL DNA was included as a positive control.