Figure 1. RT-PCR analysis of Cacna1f^wt and Cacna1f^nob2 mice.

A. Schematic representation of the location of PCR primers used. Primers RR44, 45, and 46 were used for RT-PCR reactions; primers RR50, 51, 52, and 53 were used for genomic PCR reactions. B. Agarose gel depicting RT-PCR reaction products for mRNA isolated from Cacna1f^wt and Cacna1f^nob2 mice. Regardless of the primer pair used, only a single band is detected using mRNA from Cacna1f^wt mice. Using mRNA from Cacna1f^nob2 mice, however, two bands are visible (see arrows). The relative intensities of the fluorescence signals indicate that the larger-M_r band accounts for ∼90%, and the smaller-M_r band for ∼10%, of the total mRNA.