Figure 2. Sequencing results of Cacna1f^wt and Cacna1f^nob2 mice.

A. Sequencing results from the larger-M_r, more intense band. Sequences are given for the DNA (small letters) and corresponding protein (capital letters); the sequence corresponding to wild type Ca_v1.4 protein is highlighted in grey. The ETn transposable element is inserted after the ninth Ca_v1.4 amino acid, and encodes an in-frame stop codon (highlighted in red) which is predicted to result in truncation of the Ca_v1.4 protein after only 25 amino acids. The underlined ETn sequence highlights a repetitive sequence (compare to the beginning of the ETn sequence, beginning at amino acid number 4). B. Same as A., but for the smaller-M_r, less intense band. Note that the inserted ETn element is shorter, and the in-frame stop codon is missing. C. Alignment of the predicted N-terminal amino acid sequences of wild type Ca_v1.4 protein and Ca_v1.4 protein encoded in the smaller-M_r band. A region of approximately 22 amino acids differs in the N-termini of the two clones.