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. 2008 Jun 27;4(6):e1000116. doi: 10.1371/journal.pgen.1000116

Figure 3. Promoter methylation and gene expression compared between ES cells and pMEFs.

Figure 3

(A) Promoter methylation patterns in ES cells (red bars), early passage pMEFs (pMEFs-P1, light blue bars), late passage pMEFs (pMEFs-P5, dark blue bars) and sperm (yellow bars). Candidate promoter regions were identified by the meDIP screen and validated by Sequenom analysis. The number of differentially methylated CpGs analysed for each gene are given in brackets. (B) Gene expression differences between ES cells and pMEFs (P1) as determined by quantitative RT-PCR analysis. The x-axis gives the log-fold expression difference between the cell types (i.e., log [ES/pMEF]). Three reference genes (Dynein, Rsp23 and Hdac10-11) were used for normalization between cell types.