Figure 3. PPARγ target gene expression and PPARγ activity are increased in βERKO mice.

A–E) Analysis of Lpl, PEPCK, CD36, RBP4 and adiponectin mRNA expression levels in gonadal fat from HFD-fed wt and βERKO mice. Real-time quantitative RT-PCR studies were carried out using total RNA prepared from gonadal fat isolated from HFD-fed wt and βERKO mice (n = 3 per group). For details, see Materials and Methods and supplemental data. * p<0.05 vs. wt-control. F) Adiponectin levels measured in serum isolated from fasted wt and βERKO mice (n = 10 per group); * p<0.05 vs. wt-control. Values represent means±SEM. G) Nuclear fractions isolated from gonadal fat from HFD-fed wt and βERKO mice (n = 3 and n = 4, respectively) were incubated with ³²P-labeled PPRE and analyzed by EMSA, as described in Materials and Methods. Real-time quantitative RT-PCR studies for PPARγ mRNA expression in gonadal fat were performed. Additionally 20 µg of the nuclear fraction used in EMSA assay were analyzed in Western Blot using PPARγ-specific antibody.