Figure 6. Systemic insulin sensitivity and glucose tolerance are improved in βERKO mice.

A+B) Oral glucose tolerance test (OGTT) with 2 g/kg body weight glucose and subsequent glucose analysis from the tail vein was performed as described in Materials and Methods. The area under the curve (AUC) was calculated, as indicated (n = 13 per group). * p<0.05 vs. wt-control. C+D) An Insulin tolerance test (ITT) was performed by an intraperitoneal injection of 0.5 units/kg body weight insulin and glucose analysis from the tail vein, as described in Materials and Methods. The area under the curve (AUC) was calculated, as indicated (n = 13 and n = 10 per group respectively). * p<0.05 vs. wt-control. Values represent means±SEM. E) Food efficiency (ratio of weight gain and food intake) was calculated from change in body weight gain (Figure 1G) and average food intake/ day (Table 2). Data are presented as x-fold over wt mice. ** p<0.01 vs. wt-control. F+G) O₂ consumption (VO₂), and respiratory quotient (RQ) from HFD-fed wt and βERKO mice. RQ was calculated as the ratio between CO₂ produced (VCO₂) and O₂ consumed (VO₂) using the calorimetry system described under methods. * p<0.05 vs. wt-control.