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. 2008 Jun 27;4(6):e1000108. doi: 10.1371/journal.pgen.1000108

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Foryst-Ludwig et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A–C) 3T3-L1 preadipocytes were transfected with the indicated plasmids together with pGal4-hPPARγDEF, pG5TkGL3 and renilla followed by treatment with 10 µM pioglitazone as indicated. *p<0.05 vs. pSG5+veh; # p<0,05 vs. pSG5+Pio, p<0.05 vs. ERβ+Pio, ns: not significant vs. ERβ+Pio. Values represent means±SEM of at least two independent experiments performed in triplicates. D) ChIP experiment with gonadal fat from HFD-fed wt mice and βERKO mice. IP was performed using Flag, RNA Pol II, SRC1 and TIF2 as indicated. (– no template control (NTC), + genomic DNA, input: 1% of the initial probe taken for IP). For details please see Materials and Methods.

Inline graphic — A–C) 3T3-L1 preadipocytes were transfected with the indicated plasmids together with pGal4-hPPARγDEF, pG5TkGL3 and renilla followed by treatment with 10 µM pioglitazone as indicated. *p<0.05 vs. pSG5+veh; # p<0,05 vs. pSG5+Pio, p<0.05 vs. ERβ+Pio, ns: not significant vs. ERβ+Pio. Values represent means±SEM of at least two independent experiments performed in triplicates. D) ChIP experiment with gonadal fat from HFD-fed wt mice and βERKO mice. IP was performed using Flag, RNA Pol II, SRC1 and TIF2 as indicated. (– no template control (NTC), + genomic DNA, input: 1% of the initial probe taken for IP). For details please see Materials and Methods.