Figure 6. Cavtratin, MMP-9 and EMMPRIN in Raw-264.7.

A- Top, representative zymogram of culture supernatant from Raw-264.7 macrophages. 1×10⁶ cells were incubated in a serum-free medium with Cavtratin or scrambled peptides (10 µM) for a period of 24 hours. Thereafter, culture media was collected and centrifuged for 5 minutes at 2000 rpm at 4C and subjected to gelatin zymography. Little or undetectable signal was observed for MMP-2. Middle, EMMPRIN expression levels in total cell lysates obtained from Raw-264.7 cells and representative western blot for β-actin (bottom panel). B- Quantification of MMP-9 lytic activity in the culture supernatant. * P = 0.02. C- Quantification of EMMPRIN expression levels. Data presented were normalized by β−actin expression.