Figure 7. Cavtratin, MMP-9, COX-2 and EMMPRIN in LPS-challenged Raw-264.7.

A- Top, MMP-9 activity in the culture supernatant from Raw-264.7 macrophages. 1×10⁶ cells were pre-incubated in a serum-free medium with Cavtratin or scrambled peptides (10 µM) for a period of 8 hours. Thereafter, LPS (10ng/mL) was added and the culture media was collected after 20 hours of LPS addition and centrifuged for 5 minutes at 2000 rpm at 4C and subjected to gelatin zymography. Middle top, representative western blot showing EMMPRIN expression in total cell lysates obtained from LPS-stimulated Raw-264.7 cells. Middle bottom, representative western blot for COX-2 expression in total cell lysates obtained from LPS-stimulated Raw-264.7 cells. Bottom panel, representative western blot showing β-actin expression. B- Quantification of MMP-9 lytic activity in the culture supernatant. * P<0.001. C- Quantification of EMMPRIN expression levels. Data presented were normalized by β−actin expression. D- Quantification of COX-2 expression levels. Data presented were normalized by β−actin expression. * P<0.001