Table 2. Detailed statistics of Bio-Rad 17-plex assay recoveries (two independent experiments).

Cytokines	IFN-g	TNF-a	IL-1b	IL-2	IL-4	IL-5	IL-6	IL-7	IL-8
Number of positive reads	14	13	13	13	13	13	13	13	10
Minimum	20.3	51.47	23.77	88.89	43.07	14.7	19.63	67.52	100.2
Median	98.37	91.43	114.5	106.3	101.7	115.7	92.05	168.3	104
Maximum	518.3	152.9	323.5	692.5	319.2	261.6	176.5	824	166.9
Mean	165.2	100.2	138.4	203.8	147	126.4	101.1	225	118.8
Std. Deviation	160.8	36.1	91.58	219.2	110.6	77.82	54.27	269.7	32.21
Std. Error	56.84	13.64	34.61	82.85	41.8	27.51	20.51	101.9	16.11
Coefficient of variation	97.32%	36.03%	66.15%	107.55%	75.22%	61.56%	53.67%	119.86%	27.13%

Unstimulated whole blood culture supernatant samples were spiked at different concentrations with recombinant cytokines. Samples were analysed on the Bio-plex system instrument and the recovery of each cytokine in assessed after subtraction of the endogenous levels of cytokines.