Table 3. Detailed statistics of Linco-plex assay recoveries (two independent experiments).

Cytokines	IL-1α	IL-1β	IL-1rα (−)	IL-2	IL-4	IL-5	IL-6	IL-7
Number of positive reads	6	7	4	7	7	7	5	7
Minimum	61.44	70.7	35.02	77.09	70.92	75.23	55.12	64.04
Median	87.88	93.63	82.98	87.9	92.48	112.8	78.96	77.5
Maximum	99.01	123.2	180.5	102.9	314.4	177	110.2	120.9
Mean	85.43	94.49	95.37	87.58	121.1	118.6	81.48	84.32
Std. Deviation	12.92	18.51	61.23	8.522	86.17	34.65	22.5	19.67
Std. Error	5.275	6.997	30.62	3.221	32.57	13.09	10.06	7.433
Coefficient of variation	15.13%	19.59%	64.21%	9.73%	71.15%	29.22%	27.62%	23.32%

(−) Cytokines not included in the second test.

Unstimulated whole blood culture supernatant samples were spiked at different concentrations with recombinant cytokines. Samples were analysed on the Bio-plex system instrument and the recovery of each cytokine assessed after subtraction of the endogenous levels of cytokines.