Table 4. Linco 29-plex assay reproducibility.
| Cytokines | IL-1B | IL-2 | IL-1ra | IL-4 | IL-5 | EGF | IL-6 | IL-7 | TGF−α |
| Number of experiments | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 |
| Minimum concentration | 6.46 | 1.6 | 52.92 | 5.8 | 6.05 | 1.6 | 238.7 | 3.53 | 1.6 |
| Median concentration | 7.27 | 1.6 | 59.7 | 9.36 | 6.87 | 1.6 | 258.8 | 3.99 | 1.6 |
| Maximum concentration | 9.17 | 4.33 | 79.18 | 14.09 | 8.55 | 1.6 | 304.9 | 8.69 | 1.6 |
| Mean concentration | 7.378 | 2.538 | 65.58 | 10.29 | 7.038 | 1.6 | 261.6 | 5.182 | 1.6 |
| Std. Deviation | 1.099 | 1.313 | 12.37 | 3.294 | 0.9504 | 0 | 26.13 | 2.19 | 0 |
| Std. Error | 0.4914 | 0.5872 | 5.533 | 1.473 | 0.425 | 0 | 11.69 | 0.9794 | 0 |
| Coefficient of variation | 14.89% | 51.73% | 18.87% | 32.01% | 13.50% | 0.00% | 9.99% | 42.26% | 0.00% |
Five aliquots of the same PHA-stimulated whole blood supernatant were produced and each aliquot was run on different plates and on different days.