Table 6. IFN-γ-based comparison of ELISA, LINCO 29-plex, Bio-Rad 17-plex and RnD Systems Fluorokine-MAP-13-plex assays.
| RnD Systems ELISA (pg/ml) | LINCO 29-plex (pg/ml) | Bio-Rad 17-plex (pg/ml) | RnD Systems Fluorokine-MAP (pg/ml) | |
| Whole blood supernatant | ||||
| PHA-stimulated | 1731.38 | 451.56 | 64.7 | 86.83 |
| Negative Control | 0 | 1.39 | 0 | NA |
| Mtb derived antigen 1 (supernatant A) | 1181.6 | 969.12 | 0 | NA |
| Mtb derived antigen 1 (supernatant B) | 908.83 | 950.62 | 2.14 | NA |
| Mtb derived antigen 2 (supernatant B) | 839.42 | 536.92 | 193.53 | NA |
| Mtb derived antigen 3 (supernatant D) | 489.1 | 247 | 49.08 | 30.26 |
| Mtb derived antigen 2 (supernatant C) | 235.67 | 125.97 | 104.94 | 17.65 |
| Mtb derived antigen 4 (supernatant E) | 118.9 | 133.82 | 172.19 | NA |
| Mtb derived antigen 3 (supernatant C) | 57.5 | 55.63 | 0 | 1.56 |
| Mtb derived antigen 5 (supernatant E) | 23.78 | 0.44 | 99.2 | 0.28 |
NA: Not applicable because the sample was not measured with the RnD Systems Fluorokine-MAP base kit A
Supernatant was generated using whole blood from a healthy donor (A-E) in six day assays stimulated with 5 differents Mtb derived antigens and with phytohaemagglutinin (PHA). The LINCO 29-plex measurement most closely resembled that of the RnD Systems ELISA in absolute value, and followed a similar trend, but absolute values for all three luminex assay fell below that of the ELISA.