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. Author manuscript; available in PMC: 2008 Jun 19.
Published in final edited form as: J Am Chem Soc. 2005 Oct 26;127(42):14594–14595. doi: 10.1021/ja055957m

Figure 2.

Figure 2

Repair of T(εA)T by E. coli AlkB. (A) A reaction of T(εA)T (0.1 mM) with E. coli AlkB (0.01 mM) under the standard repair conditions (50 mM MES or HEPES, 0.2 mM Fe(NH4)2(SO4)2, 2.6 mM α-ketoglutarate, 5.2 mM ascorbate) at pH 7.0 for 1 h at 37 °C led to the formation of TAT, which was resolved by HPLC and detected at A254. (B) A control experiment under the same conditions as (A) but in the absence of AlkB gave only the starting material T(εA)T. (C) The reaction mixture of (A) was analyzed by MALDI-TOF mass spectrometry. The negative molecular peak of TAT at 858.2 was detected. (D) The mixture of the control reaction in (B) was analyzed by MALDI-TOF mass spectrometry, and the negative molecular peak of T(εA)T at 882.2 was observed.