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. Author manuscript; available in PMC: 2008 Jun 19.
Published in final edited form as: J Am Chem Soc. 2005 Oct 26;127(42):14594–14595. doi: 10.1021/ja055957m

Figure 3.

Figure 3

The pH dependency and time courses for the repair of T(εA)T by AlkB. (A) T(εA)T (0.1 mM) was treated with 5 µM of AlkB at different pH, and the reactions were quenched after 5 min. Time courses for reactions between 0.1 mM of T(εA)T and AlkB at 37 °C; (B) 5 µM AlkB at pH 6.0, (C) 5 µM AlkB at pH 7.0, and (D) 10 µM of AlkB with pH 8.0 are also shown. Standard repair conditions were used with MES (for pH 4.0–6.5) or HEPES (for pH 7.0–8.5) as the buffers in 100 µL of solution.