Figure 1.

Sites of mutations in promoter region of pelE gene of E. chrysanthemi EC16. Numbering is based on the transcriptional start site determined by primer extension (data not shown). The translational start site is at +96 nt. The NdeI–MluI fragment (260 bp) was used for the binding assay and for the construction of lux fusion. The −35 and −10 regions of σ₇₀ type promoter are underlined. Two KdgR boxes are double underlined and designated as operator-1 (op-1) and operator-2 (op-2). ED-OP-1 and ED-OP-2 are deletion mutants obtained by site-directed mutagenesis at these operators (23). Boxed sequence indicates the protect region by Pir from digestion with DNaseI (−1 to +34 nt). Protect region by KdgR from DNaseI was shown with overhead line (+6 to +55 nt).