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DNA-binding assay using promoter region of pelE. NdeI–MluI fragment of pelE (Fig. 1) as the target DNA. Protein source was obtained by ammonium sulfate (AmS) fractionation of the sonicated extract of EC16 cells grown in minimal salts + glycerol + NaPP (A) and minimal salts + glycerol + NaPP + potato extract (B). Lanes: P, target DNA only; 1, DNA + 0–25% AmS fraction; 2, DNA + 25–40% AmS fraction; 3, DNA + 40–55% AmS fraction; 4, DNA + 55–80% AmS fraction.
