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SDS/PAGE of active fractions during purification of Pir protein. Samples were load onto a SDS/PAGE and detected by silver staining. Lanes: M, dalton marker; 1, EC16 crude extract; 2, active fraction from DEAE Sepharose Fast Flow column; 3, active fraction from HEPARIN POROS column; 4, active fraction from Superdex 200HR 10/30 column.
