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DNA-binding assay using purified Pir or KdgR onto promoter region of pelE. Labeled NdeI–MluI fragment of promoter region of pelE (30 fmol) (free, lane P) was mixed with 30 nM (lane 1), 50 nM (lane 2), 70 nM (lane 3) of purified Pir (A), and with 50 nM (lane 1), 70 nM (lane 2), 90 nM (lane 3) of purified KdgR (B).
