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Competition of binding between Pir and KdgR at promoter region of pelE. Purified Pir and KdgR are mixed with 32P-labeled NdeI–MluI fragment of pelE (30 fmol) before DNA-binding assay. (A) Increasing concentrations of KdgR (0–100 nM) were added to a constant concentration of Pir (50 nM). (B) Increasing concentrations of Pir (0–75 nM) were added to a constant concentration of KdgR (120 nM).
