Figure 9.

pelE expression in the pir mutant. Light production by EC16 (A) and K2367 (B) carrying the pelE-lux on their chromosome were measured by using Chemiluminescence detector CLD 100 (Tohoku Electric, Sendai, Japan). The specific activity of pelE-lux was expressed as cpm/ml/OD₆₀₀. Background values of this detector was about 10³ cpm/ml/OD₆₀₀. □, minimal salts + glycerol; ⋄, minimal salts + glycerol + NaPP; ○, minimal salts + glycerol + potato extract; ▵, minimal salts + glycerol + NaPP + potato extract. NaPP (2 g/l), potato extract (1%) were added into the minimal salts containing glycerol (2 g/l). All cultures were grown on shaker at 27°C.