Fig. 2.

Impact of complement 5a receptor (C5aR) targeting on ischaemia reperfusion injury-induced kidney damage. (a–e) Haematoxylin and eosin staining in transplanted (left panels) and non-transplanted kidneys (right panels). (a) 30 min cold ischaemia (CI), (b) 2 h CI without C5aRA and (c) 2 h CI in the presence of C5aRA. Small arrows depict glomeruli and large arrows indicate regions of haemorrhage. Figures are reduced from an original magnification of 20×. (d) Quantification of tissue damage in transplanted (left panel) and non-transplanted (right panel) kidneys. A tissue damage score was determined on a scale of 0–3 (none, mild, moderate and severe) as outlined in Materials and methods, with a maximum possible score of 9 = severest damage being attainable. (e) Spatial distribution of damage in transplanted (left panel) and non-transplanted kidneys (right panel). (f) Relative kidney damage shown as a composite damage score calculating the product of each individual damage score with its corresponding area of damage. All values were determined 72 h post-transplantation (n = 6–12).