Fig. 3.

Impact of complement 5a receptor (C5aR) targeting on ischaemia reperfusion injury-induced tubular apoptosis. (a–c) Transferase-mediated dUTP nick-end labelling (TUNEL)-positive tubular epithelial cells in transplanted (left panels) and non-transplanted kidneys (right panels). (a) 30 min cold ischaemia (CI). (b) 2 h CI without C5aRA. (c) 2 h CI in the presence of C5aRA. TUNEL-positive cells in the transplanted kidneys of groups a–c animals are depicted by arrows (left panels). Figures are reduced from an original magnification of 20×. (c, d) Quantification of apoptosis in tubular epithelial cells of transplanted (c) and non-transplanted (d) kidneys (number of TUNEL-positive cells/100 tubular cells in each of five high-power fields). Groups were compared by one way analysis of variance to determine statistical differences between treatment groups (see Material and methods). All values were determined 72 h post transplantation (n = 6–12).