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. Author manuscript; available in PMC: 2008 Jun 19.
Published in final edited form as: J Biol Chem. 2006 Mar 13;281(19):13596–13603. doi: 10.1074/jbc.M512787200

FIGURE 2. Stopped-flow kinetic analysis of Ku binding duplex DNA substrates.

FIGURE 2

A, kinetics of Ku binding a 75-bp duplex DNA. Kinetic traces were measured at a concentration of 25 nM Ku and 100 nM duplex DNA. Binding reactions were performed in the presence of 2.5 mM DTT (top trace) and in the absence of DTT (bottom trace). Measurements taken in the presence of DTT fit a single exponential decay, and in the absence of DTT, fit a double exponential decay. Each trace is the average of 12–14 individual shots. The residual values obtained for the fits are presented at the bottom of the panel. B, kinetics of Ku binding to the 21/34 hairpin DNA. Reactions and analyses were performed exactly as described in A.

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