Table 1.
Primary structures of high affinity cocaine-displaceable class I RNA aptamers
| Aptamer | Structure |
|---|---|
| 01 | ACGUUGAGUACAACCCCACCCCGUUCACGGUAGCCCUGUA |
| 05 | GCUACAGUACAACGGGCCGUGUGGAAUACACCGACAAGG |
| 06 | UCCACCGAUCUAGAUGAUCCAGGCACCCGACCACCACCUC |
| 07 | GCUUGUGGACCAAGAAGCAACCAGUCACCGUUGCCCC |
| 09 | CAACAGUCCUGUGUCCGUUGAAUCCUCUAGAUCCAGGGUG |
| 11 | GGACCCCCCACAGCAAGUUUGCCGGCGACCGCGUUCUUG |
| 13 | CUUGCCACUCCUGUCUAGCUGGCGUAGACCGCGCAGAAAG |
| 14 | GCUAGUAGCCUCAGCAGCAUAGUUUCGCCGCUAUGCAGUA |
| 16 | UAGCAUAAUGUGGAGCGUUGACCGGACCUCUCCAGUCGUA |
| 18 | UGGACUACGCACCCGCUAGUCCGUCCAAGAACUGUGCG |
| 19 | UUCUGUUCCGACCAAUUGAAUAGUCACCGUGAUGAUUUGA |
| 20, 21 | GAUGCCAGCGCGCAUUCUUCACCGAAGUACGUAUCCACG |
| 22 | UUCGCCGCUGCACUCUCGCAGCACUGGUCGGGAUGUGUC |
| Consensus | UUCACCG | ||||||
| Position | 1 | 2 | 3 | 4 | 5 | 6 | 7 |
| Consensus | U | U | C | A | C | C | G |
| Frequency | 9 | 9 | 9 | 10 | 14 | 13 | 14 |
After nine rounds of selection, the RNA pool was reverse-transcribed, ligated into pGEM-3Z (Promega), and transformed into E. coli strain JM109. Twenty-three clones were identified and sequenced. A 7-nt consensus region was found in 14 clones consisting of UUCACCG. Listed are the positions, nucleotides, and frequencies of the consensus region.