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HPLC of products of hydrolysis of Ins1 [32PO4],3,4,5,6-P5 by SopB. Recombinant SopB (0.6 μg) was incubated with Ins 1[32PO4],3,4,5,6-P5 in 50 mM Mops (pH 7.0), 10 mM EDTA, and 1 mM DTT for 30 min at 37°C. HPLC on Adsorbosphere SAX was performed as described (10) by using 3H-labeled internal standards of Ins 1,4-P2, Ins 1,3-P2, Ins 3,4-P2, Ins 1,3,4-P3, and Ins 1,3,4,5-P4.
