Table 1.
Substrate | nmol/min/mg protein |
---|---|
PtdIns 3-P | 47 |
PtdIns 3,4-P2 | 51 |
PtdIns 3,4,5-P3 | 68 |
Ins 1,3,4-P3 | 9.1 |
Ins 1,4,5-P3 | 13 |
Ins 1,3,4,5-P4 | 24 |
Ins 1,3,4,5,6-P5 | 4.5 |
Assays were performed with 10 μM substrate in 50 mM Mops (pH 7.0), 10 mM EDTA, and 1 mM DTT.