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. Author manuscript; available in PMC: 2008 Jun 20.
Published in final edited form as: Oncogene. 2007 Apr 2;26(42):6133–6140. doi: 10.1038/sj.onc.1210436

Figure 2. Reciprocal expression of mir-29b and Mcl-1.

Figure 2

Panel a: Total cellular protein from immortalized non-malignant H69 cells and malignant KMCH cells was probed using a Mcl-1 antibody. Actin was used as a loading control. Panel b: Northern blot for mir-29b using RNA enriched for small RNAs from H69 and KMCH cells. Note that both precursor and mature mir-29b are decreased in KMCH cells. As a loading control, an RNA probe against the small housekeeping RNA U6 (106 nt) was used. Panel c: Quantitative RT-PCR for mir-29b using total RNA from H69 and KMCH cells. Results expressed as copies of mir-29b per copy of Z30 RNA (mean +/− SEM; p < 0.05).