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. Author manuscript; available in PMC: 2008 Jun 20.
Published in final edited form as: Oncogene. 2007 Apr 2;26(42):6133–6140. doi: 10.1038/sj.onc.1210436

Figure 4. Effect of the putative mir-29 binding site derived from the Mcl-1 3’UTR on luciferase expression.

Figure 4

Panel a: Alignment of mir-29b with the insert derived from the Mcl-1 3’UTR. Note the complementarity at the 5’ end of mir-29b, where the crucial seed region is located. A single-base mutant insert was also synthesized, as shown. Inserts were cloned into the 3’ UTR of the p-Mir-Report vector. Panel b: Luciferase activity in HeLa cells transiently transfected with the luciferase construct alone, or cotransfected with an expression plasmid for mir-29b (p29b). Luciferase vectors were parental (pLuc), luciferase with the Mcl-1-derived 3’UTR insert (pLuc-Mcl-1 3’UTR), or luciferase with the mutated insert (pLuc-Mutant 3’UTR). Mean ± SEM, *p<0.01.