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. 1998 Nov 24;95(24):14071–14075. doi: 10.1073/pnas.95.24.14071

Table 2.

Survival rates of yeast strains grown under different oxidative stress conditions

Treatment T50, h
−Δ% survival per h
NM WT OP NM WT OP
H2O2 3.5 6.3 6.9 14.3 7.9 7.2
Paraquat 8.1 16.5 17.6 6.2 3.0 2.8
AAPH 11.0 20.2 21.3 4.5 2.5 2.3

The same amount of cells of each strain from late log phase was diluted 1:100 into a buffer containing PBS, 2% glucose, and 0.17 mM methionine in the presence or absence of H2O2 (2 mM). At each time point, an aliquot from each culture was taken and plated on minimal medium plates without leucine and the colonies were counted. Each survival rate point represents the number of colonies grown when H2O2 was present in relation to the number of colonies observed when growth was performed without H2O2 in the medium. Wild-type parental strain (WT). msrA null mutant strain (NM). Overproducer strain (OP). T50 is defined as the incubation time (hours) in culture needed to achieve 50% killing.