Table 3.

MsrA activity in Molt-4 cells

Enzyme	Specific activity (pmol/min/mg protein) on dabsyl-MetO
Extract of Molt-4 cells transfected with pcDNA 3.1	11
Extract of Molt-4 cells transfected with pcDNA 3.1 harboring the msrA cDNA	53

Molt-4 were stably transfected with either pcDNA 3.1 or pcDNA 3.1 harboring the msrA bovine cDNA. After disruption of cells in 25 mM Tris⋅HCl (pH 7.5) by sonication, enzyme activity was measured. The details about the transfection protocol and the assay for MsrA activity are described in Materials and Methods.