Figure 5.

Effect of overexpression of PTP1B or TCPTP on differentiation of K562 cells. K562 cells were transiently transfected with pcDNA3 (control vector), pcDNA3-PTP1B, or pcDNA3-TCPTP expression plasmids. An additional aliquot of control vector-transfected K562 cells were further treated with 10 μM CGP57148 for 48 h. Lysates from each condition were analyzed by immunoblotting with anti-PTP1B antibody (Top Left), and the blot was stripped and reprobed with anti-TCPTP antibody (Middle Left). This blot also was probed with anti-α-globin antibody (Bottom Left). The expression and activation status of endogenous p210 bcr-abl were analyzed by immunoblotting with anti-pTyr antibody (Bottom Right) and the blot was stripped and reprobed with anti-Abl antibody 8E9 (Top Right). Immunoblots were visualized by ECL.