Table 2.

Methylation-specific polymerase chain reaction (MSP) primers

Gene	Forward primer (5′−3′)	Reverse primer (5′−3′)
TAP-1 (unmeth)	GTA AGT TAG TTT TAG AAG GAG GTG T	ATC CTA ACC TAA AAC TCT CAA CAT C
TAP-1 (meth)	GTA AGT TAG TTT TAG AAG GAG GCG T	ATC CTA ACC TAA AAC TCT CGA CGT
TAP-2 (unmeth)	TTT TTT AAA ATA TGT TTG GAG GTT G	CCA ACA AAT AAC ACC TAT CAA TTT ACA
TAP-2 (meth)	GTT TTT TAA AAT ATG TTC GGA GGTC	AAC AAA TAA CGC CTA TCA ATT TAC G
LMP-7 (unmeth)	TTT TGA TTT GTT TTT TAT TAG ATG G	CCT TTC TCT CTA TAC ACT TTA AAC ATT
LMP-7 (meth)	TTT TTC GAT TCG TTT TTT ATT AGA	CCT TTC TCT CTA TAC ACT TTA AAC GTT

LMP, low-molecular-mass protein; meth, methylated; TAP, transporter associated with antigen processing; unmeth, unmethylated.