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CpG-DNA-dependent proliferation in wild-type (WT) and Bruton's tyrosine kinase-deficient (Btk–/–) splenic B cells. Purified B220+ cells (2 × 105) were cultured with CpG-DNA, lipopolysaccharide (LPS) or non-CpG-DNA and assayed at different time-points. Proliferation in WT (a) and Btk–/– (b) purified B cells was determined using the WST-1 assay kit.
