Figure 2.

NOD2 does not influence the induction of IL-6 and IL-10 stimulated by TLRs. MNC isolated from five healthy volunteers were preincubated for 24 hr with MDP (10 μg/ml), and subsequently stimulated for another 24 hr with LPS (1 ng/ml) or Pam3Cys (10 μg/ml). Concentrations of IL-6 (a) and IL-10 (b) were measured by enzyme-linked immunosorbent assay. Data are presented as mean ± SD, and compared by Wilcoxon paired test (n = 5, all differences not significant).