Table 1.
Binding of p127–142 and its analogues with notably reduced binding capacity to I-Ad
| Peptide | IC50 ratio | IC50 |
|---|---|---|
| P127-142 | 1·0 | 3·5 ± 7·07 × 10−6 |
| 1·0 | 8·5 ± 2·12 × 10−6a | |
| Y131F | 2·6 | 9·0 ± 0·0 × 10−6 |
| L134A | 5·3 | 4·5 ± 0·71 × 10−5 |
| L134D | > 28 | > 1·0 ± 0·0 × 10−4 |
| S136Y | > 28 | > 1·0 ± 0·0 × 10−4 |
| G139Y | 11·4 | 4·0 ± 0·0 × 10−5 |
The binding of 34 analogues was measured. Analogues other than those shown in the table did not exhibit a notable reduction (IC50 < 2·5) in their binding (data not shown). IC50 values are expressed in nm (mean ± standard error of the mean) for two independent experiments. IC50 ratios are presented in comparison with p127–142.
a
The control IC50 value for alanine mutants.
IC50, the peptide concentration that prevented binding of 50% of the labelled peptide.