Table 1.
Peptide | IC50 ratio | IC50 |
---|---|---|
P127-142 | 1·0 | 3·5 ± 7·07 × 10−6 |
1·0 | 8·5 ± 2·12 × 10−6a | |
Y131F | 2·6 | 9·0 ± 0·0 × 10−6 |
L134A | 5·3 | 4·5 ± 0·71 × 10−5 |
L134D | > 28 | > 1·0 ± 0·0 × 10−4 |
S136Y | > 28 | > 1·0 ± 0·0 × 10−4 |
G139Y | 11·4 | 4·0 ± 0·0 × 10−5 |
The binding of 34 analogues was measured. Analogues other than those shown in the table did not exhibit a notable reduction (IC50 < 2·5) in their binding (data not shown). IC50 values are expressed in nm (mean ± standard error of the mean) for two independent experiments. IC50 ratios are presented in comparison with p127–142.
The control IC50 value for alanine mutants.
IC50, the peptide concentration that prevented binding of 50% of the labelled peptide.