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. 2008 Mar;123(3):348–357. doi: 10.1111/j.1365-2567.2007.02683.x

Figure 4.

Figure 4

Inhibition of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) protein expression by carboxybutyrylated glucosamine (CGlcN). RAW264.7 cells were pretreated with different concentrations of CGlcN for 1 hr and stimulated with LPS (1 µg/ml) for another 24 hr. Equal amounts of protein in the cell lysates were electrophoresed and the protein expression levels of iNOS and COX-2 were determined using specific antibodies for iNOS and COX-2. Respective protein levels of actin were used to confirm the equal amounts of protein used for electrophoresis. BLK, LPS non-stimulated; LPS, LPS-stimulated but sample non-treated cells.