Figure 8.

Inhibition of lipopolysaccharide (LPS)-induced protein expression of I-κB kinase (IKK), I kappa B alpha (IκBα) and NIK by carboxybutyrylated glucosamine (CGlcN). RAW264.7 cells were pretreated with different concentrations of CGlcN for 1 hr and then stimulated with LPS (1 µg/ml) for another 24 hr. Equal amounts of protein in the cell lysates were electrophoresed and the expression levels of IKK, IκBα and NIK protein were determined using specific antibodies: Respective protein levels of actin were used to confirm that equal amounts of protein were used for elctrophoresis. BLK, LPS non-stimulated; LPS, LPS-stimulated but sample non-treated cells.