Figure 5.

Translesion replication is reduced in a large gap, but it requires the β subunit. (A) Translesion replication was performed with pol III holoenzyme on substrate GP21 (22-nt gap) side-by-side with substrate GP21-L (350-nt gap) as described in Materials and Methods. B shows translesion replication using a different gapped plasmid with a large gap (GP31-L), using pol III* (1.5 nM), or using reconstituted pol III* (10 nM; Pol III* R), in the absence or the presence of purified β subunit (40 nM). The extents of translesion replication are indicated at the bottom of each lane.