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. 1998 Nov 24;95(24):14118–14123. doi: 10.1073/pnas.95.24.14118

Figure 4.

Figure 4

The hfq gene is responsible for RBA. (A) Mobility-shift assay of the ompA mRNA-binding activity of MC4100 strain (hfq+), its derivatives AM111 (hfq1) and AM112 (hfq2), and hfq1 strain provided with the plasmid pHFQ607, containing the hfq gene. Cells were grown in Mops-acetate medium, and 10 μg of S30 extracts were used for the RNA–protein complex formation. (B) Western blot analysis of the same extracts as in A tested with Hfq-specific antibodies, as described in Materials and Methods.