Table 1.
Half-lives of ompA and lpp mRNAs (min) in Mops/acetate and LB
Strain | Mops/acetate
|
LB
|
Regulation
|
|||
---|---|---|---|---|---|---|
ompA | lpp | ompA | lpp | ompA | lpp | |
Wild type | 7 ± 0.9 | 17 ± 2.8 | 14 ± 0.3 | 21 ± 4.1 | 2.0 | 1.2 |
hfq1 | 43 ± 0.6 | 21 ± 3.9 | 25 ± 1.7 | 26 ± 0.6 | 0.6 | 1.2 |
hfq1/pHFQ607 | 9 ± 0.8 | 22 ± 2.5 | 19 ± 2.1 | 28 ± 4.5 | 2.1 | 1.3 |
hfq2 | 5 ± 0.9 | 20 ± 4.3 | 18 ± 0.1 | 26 ± 2.1 | 3.6 | 1.3 |
rpoS | 8 ± 1.0 | 22 ± 1.7 | 24 ± 3.9 | 22 ± 1.4 | 3.0 | 1.0 |
Half-lives of the ompA and lpp mRNAs as the average of two determinations by Northern blotting and subsequent quantification by PhosphorImaging. The relative changes in half-lives are shown as fold regulation. The wild-type strain MC4100 and the mutant strains are described in Materials and Methods.