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(A) Correlation between the actin-dependent MgATPase activities and concentration of 3xAsp myosin in the reaction mixture. The activities were measured in the presence of 24 μM actin at 30°C. •, unphosphorylated form; ○, phosphorylated form. (B) Fold of activation by RLC phosphorylation on the concentration of 3xAsp myosin in the reaction mixture.
