Table 1.

Steady–state actin-dependent ATPase activities

Myosins	Actin-dependent MgATPase		Fold activation by RLC phosphorylation
	No kinase treatment	Kinase treatment
Wild-type	0.13	0.71	5.5
	0.10	0.60	6.0
MyΔC1225	0.57	0.81	1.4
	0.55	0.76	1.4
MyΔC1528	0.46	0.74	1.6
	0.31	0.38	1.2
S1	0.89	0.78	0.9
	1.20	1.20	1.0
3xAsp	0.23	0.57	2.5
	0.33	1.10	3.3

Activities were measured in the presence of 24 μM actin and are shown as P_i liberated per head per s. Activities of wild-type and 3xAsp myosins were measured at a myosin concentration of 100 μg/ml. Data were from assay of two independently prepared samples.