Table 2.
Steady–state actin-dependent ATPase activities of chimeric myosins
| Myosins | Actin-dependent MgATPase
|
Fold activation by RLC phosphorylation | |
|---|---|---|---|
| No kinase treatment | Kinase treatment | ||
| Wild-type | 0.11 | 0.85 | 7.7 |
| Chimera A | 1.9 | 2.3 | 1.2 |
| Chimera B | 1.7 | 2.0 | 1.2 |
Activities were measured in the presence of 24 μM actin and 85 μg/ml myosin and are shown as Pi liberated per head per s.