Figure 1.
Analysis of pools after the third round of ribosome display. Pools of selected RNA were translated in vitro in the presence of [35S]methionine, and translation mixtures containing 0 and 1 μM GCN4(7P14P) peptide antigen as inhibitor were analyzed by RIA as described in Methods. Each bar represents the average of three samples. The ribosomal complexes were enriched on immobilized peptide antigen in the presence (experiment A) or in the absence (experiment B) of 2% sterilized milk during selection (for details, see text).