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The E. coli and mouse Tpt1 homologs have phosphotransferase activity. Partially purified phosphotransferase from yeast (Tpt1; A), E. coli (KptA; B), and mouse (mTpt1; C) were incubated with ligated tRNAPHE substrate and 1 mM NAD, as indicated. Portions of the mixtures were subsequently incubated with calf intestinal phosphatase (CIP), or yeast cyclic phosphodiesterase (CPDase), as shown, and samples were applied to TLC plates to resolve products.
