Figure 6. Virulence of WR or VVΔE3L after infection of ISG15+/+ and ISG15−/− mice.
A–B. Mice were inoculated i.p with purified VACV (2×107 PFU/mouse) or VVΔE3L (108 PFU/mouse). Mice were individually weighed daily, and mean percentage weight loss of each group (n = 12) was compared with the weight immediately prior to infection. C–D. Survival rate after i.p inoculation with both viruses. Dead animals were scored daily and represented as the percentage of surviving animals. P≤0.01 in all experiments. E. IL-6 measured by ELISA from serum collected at 3 hpi. Results represent the mean±SD of pooled samples from 6 mice.